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371.
G.V. Carbonell B.A.L. Fonseca L.T.M. Figueiredo A.L.C. Darini R.M. Yanaguita 《FEMS immunology and medical microbiology》1996,16(3-4):299-307
Abstract Cytotoxins have been implicated in the pathogenesis of bacterial infections. In this study, the influence of different culture conditions was evaluated on cytotoxin production by Serratia marcescens . Parameters such as culture media, incubation temperature, starting pH of culture medium, aeration, anaerobiosis, carbon sources, iron concentration in the culture media, and release of cell-bound toxin by polymyxin B were investigated. The data suggest that this cytotoxin is predominantly extracellular and is not induced by iron limitation. Aerobic culture with shaking resulted in higher cytotoxicity than static aerobic or anaerobic culture. Bacteria grown in glucose, sucrose or galactose were more cytotoxic than those grown in inositol or maltose. The culture conditions that were identified as optimal for cytotoxin production by Serratia marcescens were incubation temperature ranging from 30 to 37°C, in medium adjusted to pH 8.5, with shaking. This work will contribute to further studies on the identification of this cytotoxic activity. 相似文献
372.
Carbonell Ellgutter Jennifer A. Ehrich Dorothee Killengreen Siw T. Ims Rolf A. Unnsteinsdttir Ester R. 《Oecologia》2020,192(2):403-414
Oecologia - Identifying resources driving long-term trends in predators is important to understand ecosystem changes and to manage populations in the context of conservation or control. The arctic... 相似文献
373.
374.
The entomopathogenic fungus Paecilomyces farinosus showed proteolytic activity in both solid and semi-liquid culture with gelatin as sole N and C source. Semi-liquid cultures
were used to characterise proteases. Zymography of crude culture filtrates showed several bands of gelatin degradation in
electrophoresis gels. Gel filtration chromatography of these filtrates revealed two peaks of proteolytic activity. Ion-exchange
absorption eliminated gelatin from culture filtrates while retaining activity and was used to semipurify P. farinosus proteases. Semipurified culture filtrates had basic pH (8.5 approx.) optimum for proteolytic activity. Treatment of these
filtrates with effectors revealed that P. farinosus proteases are serine proteases containing sulphydryl groups. Isoelectrofocusing combined with zymography revealed the presence
of several active basic isoforms. Larvae of the lepidopteran Galleria mellonella showed cuticle damage and protein release 1h after incubation with semipurified extracts of P. farinosus. These results indicate that proteolytic enzymes could be involved in insect host penetration by P. farinosus. 相似文献
375.
Juliana Morini Küpper Cardoso Perseguini Gliciane Micaele Borges Silva Jo?o Ricardo Bachega Feijó Rosa Rodrigo Gazaffi Jéssica Fernanda Mar?al Sérgio Augusto Morais Carbonell Alisson Fernando Chiorato Maria Imaculada Zucchi Antonio Augusto Franco Garcia Luciana Lasry Benchimol-Reis 《Genetics and molecular biology》2015,38(1):67-78
Because of the continuous introduction of germplasm from abroad, some collections
have a high number of accessions, making it difficult to explore the genetic
variability present in a germplasm bank for conservation and breeding purposes.
Therefore, the aim of this study was to quantify and analyze the structure of genetic
variability among 500 common bean accessions to construct a core collection. A total
of 58 SSRs were used for this purpose. The polymorphism information content (PIC) in
the 180 common bean accessions selected to compose the core collection ranged from
0.17 to 0.86, and the discriminatory power (DP) ranged from 0.21 to 0.90. The 500
accessions were clustered into 15 distinct groups and the 180 accessions into four
distinct groups in the Structure analysis. According to analysis of molecular
variance, the most divergent accessions comprised 97.2% of the observed genetic
variability present within the base collection, confirming the efficiency of the
selection criterion. The 180 selected accessions will be used for association mapping
in future studies and could be potentially used by breeders to direct new crosses and
generate elite cultivars that meet current and future global market needs. 相似文献
376.
The heat stability of the precipitin reactants of normal chicken sera was determined. Type A sera were found to be more resistant to heating than those of type B. This difference may be used for an easy and reliable classification of both types of chicken sera. 相似文献
377.
Tratamiento con equinocandinas en un paciente crítico sometido a técnica continua de reemplazo renal
Gerardo Aguilar José Antonio Carbonell Carlos Ferrando Rafael Badenes Francisco Javier Belda 《Revista iberoamericana de micología》2012,29(2):85-89
BackgroundCritically ill patients with invasive candidiasis (IC) often suffer renal failure, which sometimes requires continuous renal replacement techniques (CRRT). Echinocandins are the first line treatment for IC in critically ill patients with mild or severe illness. Their elimination during CRRT should be negligible due to their pharmacokinetic and pharmacodynamic (PK/PD) profile, and dose adjustment are not needed, as suggested by the few reported clinical studies.Clinical caseThis is the case of a 66 year old male who underwent surgery due to peritonitis secondary to intestinal suture dehiscence. The patient was admitted to ICU with septic shock symptoms and multiple organ dysfunction syndrome (MODS), and CRRT was started. Anidulafungin was prescribed at the usual dosage due to the IC risk factors present, and the observation of yeasts in the peritoneal fluid. Anidulafungin was selected due to the hepatic failure suffered by the patient. An isolate of Candida albicans susceptible to fluconazole was cultured from peritoneal fluid and rectal exudates. However, anidulafungin was maintained due to the MODS and observing the clearance of fluconazole during CRRT. The patient's condition improved favourably, being moved to the surgical ward 20 days after the surgery.ConclusionsEchinocandins, due to their PK/PD profile, could be safely given at usual doses to critically ill patients undergoing CRRT. However, new studies are required to strengthen this recommendation. Its extrahepatic metabolism makes anidulafungin a more attractive option among echinocandins and other antifungals when used in patients with different degrees of hepatic failure 相似文献
378.
Breed distribution of precipitin reactants (of types A and B) was encountered in: Brahma and Columbian Plymouth Rock - high strength and frequencies; White Cornish, Dark Cornish, Minorca, Barred Plymouth Rock, New Hampshire, Rhode Island Red, Catalana and Cubalaya - low to medium strength and frequencies; Australorp, White Leghorn, Polish, Sexline - no reactions. Cocks exhibited only type A reactants. Both A and B substances were detected in egg yolks. Type A substances were encountered in sera of newly hatched chicks. Their decay was slow - the mean persistence time being about 15 days. Only two young chicks among 160 tested exhibited the reactants of type B. Hormonal treatments of White Leghorn and Rhode Island Red cocks with testosterone propionate, progesterone, oestradiol benzoate or oestradiol benzoate along with progesterone failed to induce the synthesis of precipitin reactants. 相似文献
379.
Enzymes in glycolysis and the citric acid cycle in the yeast and mycelial forms of Paracoccidioides brasiliensis 总被引:2,自引:2,他引:0
Kanetsuna, Fuminori (Instituto Venezolano de Investigaciones Cientificas, Caracas, Venezuela), and Luis M. Carbonell. Enzymes in glycolysis and the citric acid cycle in the yeast and mycelial forms of Paracoccidioides brasiliensis. J. Bacteriol. 92:1315-1320. 1966.-Enzymatic activities in glycolysis, the hexose monophosphate shunt, and the citric acid cycle in cell-free extracts of the yeast and mycelial forms of Paracoccidioides brasiliensis were examined comparatively. Both forms have the enzymes of these pathways. Activities of glucose-6-phosphate dehydrogenase and malic dehydrogenase of the mycelial form were higher than those of the yeast form. Another 15 enzymatic activities of the mycelial form were lower than those of the yeast form. The activity of glyceraldehyde-3-phosphate dehydrogenase showed the most marked difference between the two forms, its activity in the mycelial form being about 20% of that in the yeast form. 相似文献
380.
Vanessa Carbonell 《Bioethics》2014,28(5):245-254
Is a painful experience less bad for you if you will not remember it? Do you have less reason to fear it? These questions bear on how we think about medical procedures and surgeries that use an anesthesia regimen that leaves patients conscious – and potentially in pain – but results in complete ‘drug‐induced amnesia’ after the fact. I argue that drug‐induced amnesia does not render a painful medical procedure a less fitting object of fear, and thus the prospect of amnesia does not give patients a reason not to fear it. I expose three mistakes in reasoning that might explain our tendency to view pain or discomfort as less fearful in virtue of expected amnesia: a mistaken view of personal identity; a mistaken view of the target of anticipation; and a mistaken method of incorporating past evidence into calculations about future experiences. Ultimately my argument has implications for whether particular procedures are justified and how medical professionals should speak with anxious patients about the prospect of drug‐induced amnesia. 相似文献